Next bus departures
| Operated by | Vehicle type | Departure time | Departure location | Trip duration | Arrival time | Arrival location | Recommended | Price and booking link |
|---|---|---|---|---|---|---|---|---|
 | Bus | Knoxville Bus Station | Gainesville Bus Stop | CheapestFastest | ||||
Greyhound Lines, Inc. + FlixBus | Bus | Knoxville Bus Station | Rosa Parks Station | No tags |
Last refresh of the prices: Today at 3:26 PM EST.
Get from Knoxville to Gainesville
The journey takes about 11 hours 20 minutes and with fares starting at just $56, it presents an excellent value for a comfortable ride.
Where are the popular stops in Knoxville and Gainesville?
Stops in Knoxville
- AKnoxville Area Transit Bus Stop, 100 Kirkwood St, Knoxville, TN 37914, USA
Stops in Gainesville
- A700 SE 3rd St, Gainesville, FL 32601
- B3509 SW Williston Rd, Gainesville, FL 32608, USA
- C101 NE 23rd Ave, Gainesville, FL 32609
Compare bus companies
Greyhound Lines offers the most comprehensive intercity bus services in the United States. They operate a modern, environmentally friendly fleet that serves more than 2400 destinations and carries nearly 16 million passengers annually. When traveling with them, you can expect free WiFi, onboard entertainment, bathrooms, power outlets and decent legroom. One of the great perks of Greyhound is that they connect big cities and smaller towns in the US, where you usually can't get to by train or plane. If you're looking for safe and affordable transportation, Greyhound is a great choice.
Frequently asked questions about traveling from Knoxville, TN to Gainesville, FL by bus
Find cheapest prices for buses
Price is for a one-way ticket for one passenger and based on past searches.
The cheapest trip from Knoxville, TN to Gainesville, FL was searched and found on Nov 27, 2025 with a price of $145To save money and be sure you have the best seat, it's a good idea to buy your bus tickets from Knoxville, TN to Gainesville, FL, as early as possible.
You can expect to pay from $145 to $246 for a bus ticket from Knoxville, TN to Gainesville, FL based on the last 2 days. You can expect to find the cheapest price for the trip at $145 which is on 2025-11-27.
