Next bus departures
| Operated by | Vehicle type | Departure time | Departure location | Trip duration | Arrival time | Arrival location | Recommended | Price and booking link |
|---|---|---|---|---|---|---|---|---|
 | Bus | Knoxville Bus Station | Gainesville Bus Stop | CheapestFastest | ||||
Greyhound Lines, Inc. + FlixBus | Bus | Knoxville Bus Station | Rosa Parks Station | No tags |
Last refresh of the prices: Today at 3:26 PM EST.
Get from Knoxville to Gainesville
The journey takes about 11 hours 20 minutes, and with fares starting at just $80, it presents an excellent value for a comfortable ride.
Where are the popular stops in Knoxville and Gainesville?
Stops in Knoxville
- AKnoxville Area Transit Bus Stop, 100 Kirkwood St, Knoxville, TN 37914, USA
Stops in Gainesville
- A700 SE 3rd St, Gainesville, FL 32601
- B3509 SW Williston Rd, Gainesville, FL 32608, USA
- C101 NE 23rd Ave, Gainesville, FL 32609
Compare bus companies
Based on 87397 reviews, the company was rated 3.5 stars on Busbud. Travellers were especially satisfied with the ticket access and the temperature but often complained with the WiFi. Greyhound ticket prices on this trip start at $80
Frequently asked questions about travelling from Knoxville, TN to Gainesville, FL by bus
What is the distance between Knoxville and Gainesville?
The distance covered from Knoxville to Gainesville is 718 km (446 miles).
What are the departure and arrival locations for buses traveling from Knoxville to Gainesville?
Buses traveling this route start their trip at 100 Kirkwood St, in Knoxville, and end it at Rosa Parks Station, in Gainesville.
Find cheapest prices for buses
Price is for a one-way ticket for one passenger and based on past searches.
The cheapest trip from Knoxville, TN to Gainesville, FL was searched and found on Nov 27, 2025 with a price of $209To save money and be sure you have the best seat, it's a good idea to buy your bus tickets from Knoxville, TN to Gainesville, FL, as early as possible.
You can expect to pay from $209 to $354 for a bus ticket from Knoxville, TN to Gainesville, FL based on the last 2 days. You can expect to find the cheapest price for the trip at $209 which is on 2025-11-27.
